high gradient fractionations

Isotopic fractionation in non‐equilibrium diffusive

Isotopic fractionation in non‐equilibrium diffusive

For high concentration gradients, the observed fractionation may be >2 per mil, whereas observed fractionations are near zero for small concentration gradients. The overall magnitude of fractionation is determined by concentration gradient, but soil gas diffusivity affects the timing of

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The Effect of Temperature and Hydrogen Limited Growth on

The Effect of Temperature and Hydrogen Limited Growth on

The range of fractionations (1.510‰ ) was typical for growth with hydrogen as the electron donor. Fractionations decreased with increasing temperature from 4060° C, and increased with increasing temperatures from 6080° C. Growth under H 2 limited conditions in a fedbatch culture revealed high fractionations of 2437‰ .

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MODELING THE ISOTOPIC FRACTIONATION OF MAGNESIUM

MODELING THE ISOTOPIC FRACTIONATION OF MAGNESIUM

to high temperature gradients produces large isotopic fractionations of Mg and Ca[1,2]. These observations were modeled using an empirical Soretbinary diffusion model. This curious phenomenon led us to attempt to understand the origins of isotopic fractionation in these high temperature systems from a more fundamental perspective.

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Isotopic fractionation of the major elements of molten

Isotopic fractionation of the major elements of molten

tope fractionations would be one in which a homogeneous starting material is placed in a temperature gradient and held for a sufficiently long time for the system to reach a steady state. New isotopic measurements of the run products of a molten basalt thermal diffusion experiment show that all the major elements except monoisotopic aluminum

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Cell Fractionation and Organelle Isolation Thermo Fisher

Cell Fractionation and Organelle Isolation Thermo Fisher

Density gradient centrifugation approaches are also effective, but these are generally only practical for largescale needs. When the goal is smallscale enrichment of mitochondria and/or extraction of mitochondrial proteins, a reagentbased microcentrifuge method is desirable.

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Exploring the molecularlevel mechanism of isotope

Exploring the molecularlevel mechanism of isotope

For high temperature silie melts, the theory offers a simple equation for calculating isotopic fractionations of all isotope systems. In their study, a unified equation for calculated isotope fractionation in thermal diffusion for isotope system M ( Δ X M) is deduced, and it is for all temperature conditions.

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Cell Fractionation: Extraction, Homogenization and

Cell Fractionation: Extraction, Homogenization and

The tube when centrifuged at high speed the various organelles migrate to an equilibrium position where their density is equal to the density of the medium. Meselson, Stahl and Vinograd (1957) used denser cesium chloride gradient for separation of a heavy DNA with 15 N from DNA with 14 N to provide evidence for semiconservative DNA repliion.

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Cell fractionation for Western Blot protocol

Cell fractionation for Western Blot protocol

The nuclei were then washed in 1ml of cytoplasmic lysis buffer to remove any contaminating cytoplasm, and resedimented. The nuclei were then lysed in 100μl of denaturing lysis buffer (2% SDS, 2M

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Isotopic fractionation in non‐equilibrium diffusive

Isotopic fractionation in non‐equilibrium diffusive

For high concentration gradients, the observed fractionation may be >2 per mil, whereas observed fractionations are near zero for small concentration gradients. The overall magnitude of fractionation is determined by concentration gradient, but soil gas diffusivity affects the timing of

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Recent Advances in HighTemperature Fractionation of

Recent Advances in HighTemperature Fractionation of

The hightemperature solvent gradient liquid chromatography system 1decanol/1,2,4trichlorobenzene/porous graphite HypercarbTM has been applied to the separation of

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Preparation of Synaptoneurosomes from Mouse Cortex using a

Preparation of Synaptoneurosomes from Mouse Cortex using a

Constituents of the bands from the discontinuous Percollgradient fractionations of homogenized mouse cortex. 3,5. Table 2. Summary of Western bolt analysis of discontinuous Percollsucrose gradient band constituents. the gradients have to be compensated for with a salt gradient. 31 SNs prepared with high concentrations of sucrose (1.40.8

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Novel Multiprotein Complexes Identified in the

Novel Multiprotein Complexes Identified in the

gradient (15 liters) of 0–500 mM NaCl in Buffer A, and 125ml anaerobic fractions were collected (120 fractions). Any remaining tightly bound proteins were eluted from the column using 1liter step gradients of 1, 1.5, and 2 M NaCl, respectively, in Buffer A (high salt washes). Second (c2) Level Column Fractionations—A subset of 37 gradient

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Chapter 3 Centrifugation

Chapter 3 Centrifugation

gradient centrifugation. Easier to They contain extracellular fluid (high in Ca and Na ions) They are continuous tubes of sarcolemmal membrane that run through (transversely) the muscle fiber. Sarcoplasmic reticulum The sarcoplasmic reticlum (SR) is the Ca store. It is a diffuse membrane structure that surrounds the sarcomere

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The Soret effect and isotopic fractionation in high

The Soret effect and isotopic fractionation in high

Apr 20, 2011 · Our approach in adapting transitionstate theory to elemental diffusion in hightemperature geochemical systems was to use the empirical data on the chemical and isotopic fractionations of

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Rapid, SingleSpin Fractionation of Serum Lipoproteins by

Rapid, SingleSpin Fractionation of Serum Lipoproteins by

lar lipoprotein fractionations using either fixedangle or swingingbucket rotors. In addition, suitable modifiion of the discontinuous gradient, and/or gradient materials, should permit further resolution of subcomponents within each lipoprotein class, for example, the resolution of LDL1 from LDL2, or HDL2, from HDL3. References 1.

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Separation and Characterization of Wheat Protein Fractions

Separation and Characterization of Wheat Protein Fractions

since 1988. Gliadins were eluted by a multistep linear gradient at 65°C, starting at 25% CH3CN and 75% water each containing 0.1% trifluoroacetic acid (TFA). The CH3CN concentration increased to 30% at 5 min, to 40% at 55 min, to at 56 min, and then returned to

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A comparison of the thermal gradient and elution methods

A comparison of the thermal gradient and elution methods

As a test of the effectiveness of the thermal gradient, comparisons have been made of fractionation by these two column methods on high molecular weight polystyrene samples. It was found that the thermal gradient method definitely provides superior resolution and reproducibility, as expected.

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The Effect of Temperature and Hydrogen Limited Growth on

The Effect of Temperature and Hydrogen Limited Growth on

The range of fractionations (1.510‰ ) was typical for growth with hydrogen as the electron donor. Fractionations decreased with increasing temperature from 4060° C, and increased with increasing temperatures from 6080° C. Growth under H 2 limited conditions in a fedbatch culture revealed high fractionations of 2437‰ .

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The desmosome is a mesoscale lipid raft–like membrane

The desmosome is a mesoscale lipid raft–like membrane

May 30, 2019 · Consistent with these calculations, sucrose gradient fractionations revealed that Dsg1(G578R) was virtually absent from lipid raft (DRM) fractions (Figure 7, A and B). Interestingly, we observed a notable reduction in plakoglobin association with DRM in cell lines expressing Dsg1(G578R), suggesting that the DSG1 mutant also recruited

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Parameterization of high magnetic field gradient

Parameterization of high magnetic field gradient

A detailed quantitative analysis of a batchwise magnetic fractionation process for malaria infected erythrocytes using high gradient magnetic fractionation columns was performed. The models applied in this study allow the prediction of capture efficiency if the initial infected cell

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Centrifugation: Theory, Sedimentation Rate, Coefficient

Centrifugation: Theory, Sedimentation Rate, Coefficient

Fractionations carried out on the basis of particle density alone are called isopycnic separations. It is to be noted that this implies that small, dense particles with low sedimentation coefficients sediment further through such a density gradient than large, light particles with high

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IJMS Free FullText Fractionation of Enriched

IJMS Free FullText Fractionation of Enriched

The conventional highpHRP fractionation utilizes fixed pH conditions with the gradient of ACN that is usually applicable for peptide fractionation. On the contrary, our pH/ACNgradientRP fractionation technique employed a simultaneous gradient of both pH (3.9–6.6) and ACN (4–24%).

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Fractionation Wikipedia

Fractionation Wikipedia

Fractionation is a separation process in which a certain quantity of a mixture (gas, solid, liquid, enzymes, suspension, or isotope) is divided during a phase transition, into a number of smaller quantities in which the composition varies according to a gradient.Fractions are collected based on differences in a specific property of the individual components.

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Recent developments in polyolefin fractionations

Recent developments in polyolefin fractionations

each other in terms of separation characteristics. For adsorption based fractionations high temperature high performance liquid chromatography (HTHPLC) seems to be preferred over temperature gradient interaction chromatography (TGIC) because of the superior efficiency and separation power however,

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Parameterization of high magnetic field gradient

Parameterization of high magnetic field gradient

Fractionations were carried out in a single pass through the columns. Cells were enumerated and differentiated in the initial samples as well as in the positive and negative fractions. >A detailed quantitative analysis of a batchwise magnetic fractionation process for malaria infected erythrocytes using high gradient magnetic fractionation

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Hydrogen exchange during cellulose synthesis distinguishes

Hydrogen exchange during cellulose synthesis distinguishes

treering cellulose in the presence of xylem water of high D abundance. The large D gradient between leaf metabolites and xylem water allowed us to distinguish effects of hydrogen exchange from possible D fractionations. The abundances of individual D isotopomers were measured using deuterium nuclear magnetic resonance spectroscopy (D NMR). The

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Preparation of Preformed Iodixanol Gradients

Preparation of Preformed Iodixanol Gradients

For many cell fractionations that are run at room temperature, the cell suspension can be included in one or more of the layers. (or Gradient Mate ) in which the low and highdensity solutions, layered in the centrifuge tube, produce a continuous gradient by controlled mixing. The tubes are rotated at a preset angle (to increase the

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Parameterization of high magnetic field gradient

Parameterization of high magnetic field gradient

A detailed quantitative analysis of a batchwise magnetic fractionation process for malaria infected erythrocytes using high gradient magnetic fractionation columns was performed. The models applied in this study allow the prediction of capture efficiency if the initial infected cell

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Recent Advances in HighTemperature Fractionation of

Recent Advances in HighTemperature Fractionation of

The hightemperature solvent gradient liquid chromatography system 1decanol/1,2,4trichlorobenzene/porous graphite HypercarbTM has been applied to the separation of

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IJMS Free FullText Fractionation of Enriched

IJMS Free FullText Fractionation of Enriched

The conventional highpHRP fractionation utilizes fixed pH conditions with the gradient of ACN that is usually applicable for peptide fractionation. On the contrary, our pH/ACNgradientRP fractionation technique employed a simultaneous gradient of both pH (3.9–6.6) and ACN (4–24%).

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Autophagy of cytoplasmic bulk cargo does not require LC3

Autophagy of cytoplasmic bulk cargo does not require LC3

Although our density gradient fractionations showed some overlap between LC3 and sequestered LDH at the early hours after cycloheximide treatment,6 they cannot really tell whether there is ever any physical association of LC3 with the phagophores that perform macroautophagic cargo sequestration. If

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Carbon isotope compositions of terrestrial C3 plants as

Carbon isotope compositions of terrestrial C3 plants as

Nov 16, 2010 · A broad compilation of modern carbon isotope compositions in all C3 plant types shows a monotonic increase in δ 13C with decreasing mean annual precipitation (MAP) that differs from previous models. Corrections for temperature, altitude, or latitude are smaller than previously estimated. As corrected for altitude, latitude, and the δ 13C of atmospheric CO2, these data permit refined

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Parameterization of high magnetic field gradient

Parameterization of high magnetic field gradient

Parameterization of high magnetic field gradient fractionation columns for appliions with Plasmodium falciparum infected human erythrocytes. Author(s) : Karl, Fractionations were carried out in a single pass through the columns. Cells were enumerated and differentiated in the initial samples as well as in the positive and negative fractions.

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Isotopic fractionation of the major elements of molten

Isotopic fractionation of the major elements of molten

tope fractionations would be one in which a homogeneous starting material is placed in a temperature gradient and held for a sufficiently long time for the system to reach a steady state. New isotopic measurements of the run products of a molten basalt thermal diffusion experiment show that all the major elements except monoisotopic aluminum

Get price
Parameterization of high magnetic field gradient

Parameterization of high magnetic field gradient

Fractionations were carried out in a single pass through the columns. Cells were enumerated and differentiated in the initial samples as well as in the positive and negative fractions. >A detailed quantitative analysis of a batchwise magnetic fractionation process for malaria infected erythrocytes using high gradient magnetic fractionation

Get price
A comparison of the thermal gradient and elution methods

A comparison of the thermal gradient and elution methods

As a test of the effectiveness of the thermal gradient, comparisons have been made of fractionation by these two column methods on high molecular weight polystyrene samples. It was found that the thermal gradient method definitely provides superior resolution and reproducibility, as expected.

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Solvent gradient fractionation of Polybutene1 resin and

Solvent gradient fractionation of Polybutene1 resin and

Jun 03, 2020 ·Ł. Introduction. Polybutene1 (PB1) resin is a semicrystalline polymer with many outstanding properties [].PB1 has four different crystalline forms (I, I′, II and III) depending on the formation conditions [,,, ].Form II, Form I′, and Form III crystals are usually formed from melt at atmospheric pressure, the melt under a high pressure, and solution crystallization, respectively.

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A Comparison of Molecular Weight Distributions for

A Comparison of Molecular Weight Distributions for

Esfandiar Mokhtari‐Nejad, Knud C. Berger, Reinhart Hammel, Klaus Lederer, Baker‐Williams fractionation of high molecular weight polyisobutylene and its evaluation by gel permeation chromatography, Die Makromolekulare Chemie, 10.1002/macp.1978.021790115, 179, 1, (159

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Cell Fractionation: Definition, Steps & Methods Video

Cell Fractionation: Definition, Steps & Methods Video

High School Chemistry: Help and Review Cell fractions can be also separated in one step using a density gradient, as shown in this figure. The cell fractions are applied to a test tube that

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Characterization of HydroxyInterlayered Vermiculite and

Characterization of HydroxyInterlayered Vermiculite and

Aug 01, 1993 · Xray diffraction, FTIR, and chemical analyses were performed on clay fractions (1–2 µm, <0.1 µm), separated by means of size fractionations and highgradient magnetic separation techniques, from a Cryorthod developed in a chloritemica schist saprolite. Weathering of large phyllosilies preexisting in the saprolite involves physical fragmentation and mineralogical

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Modeling of Elemental Isotopic Fractionations Induced By

Modeling of Elemental Isotopic Fractionations Induced By

Recent experiments by Richter et al. (1,2) found that significant isotopic fractionations of Mg and Ca (and other elements) are produced in basalt melts that are placed in high temperature thermal gradients (~ 1400 1520 degrees C). Using a simple 1D activation energy dependent probabilistic hopping model of diffusion in condensed phases, we calculate the timedependent and steadystate

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Centrifugation: Theory, Sedimentation Rate, Coefficient

Centrifugation: Theory, Sedimentation Rate, Coefficient

Fractionations carried out on the basis of particle density alone are called isopycnic separations. It is to be noted that this implies that small, dense particles with low sedimentation coefficients sediment further through such a density gradient than large, light particles with high

Get price
Parameterization of high magnetic field gradient

Parameterization of high magnetic field gradient

Parameterization of high magnetic field gradient fractionation columns for appliions with Plasmodium falciparum infected human erythrocytes. Author(s) : Karl, Fractionations were carried out in a single pass through the columns. Cells were enumerated and differentiated in the initial samples as well as in the positive and negative fractions.

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Different hydrogen isotope fractionations during lipid

Different hydrogen isotope fractionations during lipid

Jan 25, 2016 · Leaf wax δDnalkane values have shown to differ significantly among plant life forms (e.g., among grasses, shrubs and trees) in higher plants. However, the underlying causes for the differences

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The Effect of Temperature and Hydrogen Limited Growth on

The Effect of Temperature and Hydrogen Limited Growth on

The range of fractionations (1.510‰ ) was typical for growth with hydrogen as the electron donor. Fractionations decreased with increasing temperature from 4060° C, and increased with increasing temperatures from 6080° C. Growth under H 2 limited conditions in a fedbatch culture revealed high fractionations of 2437‰ .

Get price
Cell Fractionation: Extraction, Homogenization and

Cell Fractionation: Extraction, Homogenization and

The tube when centrifuged at high speed the various organelles migrate to an equilibrium position where their density is equal to the density of the medium. Meselson, Stahl and Vinograd (1957) used denser cesium chloride gradient for separation of a heavy DNA with 15 N from DNA with 14 N to provide evidence for semiconservative DNA repliion.

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CiteSeerX — CHARACTERIZATION OF HYDROXYINTERLAYERED

CiteSeerX — CHARACTERIZATION OF HYDROXYINTERLAYERED

CiteSeerX Document Details (Isaac Councill, Lee Giles, Pradeep Teregowda): AbstractXray diffraction, FTIR, and chemical analyses were performed on clay fractions (12/~m, <0.1 pm), separated by means of size fractionations and highgradient magnetic separation techniques, from a Cryorthod developed in a chloritemica schist saprolite.

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Isotopic fractionation in non‐equilibrium diffusive

Isotopic fractionation in non‐equilibrium diffusive

For high concentration gradients, the observed fractionation may be >2 per mil, whereas observed fractionations are near zero for small concentration gradients. The overall magnitude of fractionation is determined by concentration gradient, but soil gas diffusivity affects the timing of

Get price
Parameterization of high magnetic field gradient

Parameterization of high magnetic field gradient

A detailed quantitative analysis of a batchwise magnetic fractionation process for malaria infected erythrocytes using high gradient magnetic fractionation columns was performed. The models applied in this study allow the prediction of capture efficiency if the initial infected cell

Get price